Clinical Staphylococcus aureus Isolate Negative for the Sa442 Fragment

Clinical Staphylococcus aureus isolate negative for the Sa442 fragment.

The Sa442 DNA fragment, originally described by Martineau et al. (3), is a popular DNA target for identification of Staphylococcus aureus by PCR (2–6). Several PCR-based assays targeting this DNA fragment alone or in combination with a mecA PCR for identification of a methicillin-resistant S. aureus have been described (3, 4). Apart from having been established as being unique for S. aureus, th...

Identification of 2,600 clinical methicillin-resistant Staphylococcus aureus strains in The Netherlands yielded sporadic cases of strains negative for the species-specific Sa442 gene fragment.

Genome Sequence of the Clinical Isolate Staphylococcus aureus subsp. aureus Strain UAMS-1

We report here the draft genome sequence of Staphylococcus aureus subsp. aureus strain UAMS-1. UAMS-1 is a virulent oxacillin-susceptible clinical isolate. Its genome is composed of 2,763,963 bp and will be useful for further gene expression analysis using RNA sequencing (RNA-seq) technology.

Sporadic cases of Staphylococcus aureus organisms negative for a species-specific 442-base pair chromosomal fragment.

Staphylococcus aureus has been well known as a major pathogen for many years, causing a variety of nosocomial infections. The rapid and reliable detection of methicillin-resistant S. aureus (MRSA) organisms is of utmost importance to prevent the spread of infection. Species identification is primarily based on biochemical characteristics of cultured organisms, but to increase accuracy of identi...

Emergence of a clinical daptomycin-resistant Staphylococcus aureus isolate during treatment of methicillin-resistant Staphylococcus aureus bacteremia and osteomyelitis.

The emergence of a clinically daptomycin-resistant Staphylococcus aureus isolate occurred during treatment of methicillin-resistant S. aureus bacteremia and probable vertebral osteomyelitis. The breakthrough isolate was indistinguishable from pretreatment daptomycin-susceptible isolates by pulsed-field gel electrophoresis. Daptomycin nonsusceptibility was confirmed by MIC and time-kill curve an...